RNA-seq analysis of Piezo1-deficient muscle satellite cells

Muscle satellite cells (MuSCs), skeletal muscle-resident stem cells, are crucial for regeneration of myofibers. Mechanical cues are thought to be important for activation and proliferation of muscle satellite cells, but the molecular entity that senses biophysical forces in MuSCs remains to be elucidated. In this study, we identified PIEZO1, a mechanosensitive ion channel that is activated by membrane tension, as a critical determinant for myofiber regeneration. We investigated gene profiles of Piezo1-deficient MuSCs to understand the role of PIEZO1 during myogenesis. Our results suggest that PIEZO1 governs the cytoskeletal reorganization to regulate cellular events in MuSCs (i.e., activation, cell-division, and proliferation) during skeletal muscle regeneration. Wild type or Piezo1-deficient satellite cells were isolated by FACS (fluorescence-activated cell sorting) as a population of VCAM1+ve, CD31-ve, CD45-ve, Sca1-ve cells. Total RNA samples were isolated from MuSCs cultured for 3 days.