Proteomic data (FCS files, flow cytometry) for expanded regulatory T cells in autoimmune polyendocrine syndrome type 1

The deposit contains flow cytometric characterization and Treg suppression assay data for the paper "Single cell characterization of blood and expanded regulatory T cells in autoimmune polyendocrine syndrome type 1", published in iScience in 2024.

1. Flow cytometry characterization of expanded Tregs
Tregs were isolated from whole blood from 17 APS-1 patients and 14 healthy controls and expanded in vitro for 14 days and then frozen. These were then characterized by flow cytometry. Please see the publication for details.
2. Tregs suppression assay
Thawed PBMCs from 15 APS-1 patients and 15 healthy controls were used to obtain responder T cells (Tresp). Cells were then cultured to a concentration of 1x106 cells/ml and rested overnight. Next day, Tresp cells were stained with the CellTrace Violet Cell Proliferation Kit. To assess the suppressive capacity, recovered expanded Tregs were activated and co-cultured at different ratios for 5 days at 37℃ and 5% CO2. Cells were harvested and stained for flow cytometry.