Localization of Mam1 to kinetochores during meiosis I in wild type and various mutant strains.

Data obtained from the microscopic screen are presented above. A hundred cells in triplicate were scored for each strain after transfer to SPM for 5–8 h. The average percentage of cells showing Mam1-GFP expression and percentage of GFP-positive cells having co-localization of Mam1-GFP foci with Cep3–RFP or Mtw1-RFP are indicated. Genotypes of Cep3-RFP and Mtw1-RFP strains are indicated in normal type and boldface respectively.