The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor - Behavioral data from perturbation experiments

This dataset features the behavioral data from the E-PG, AOTU, and control Shibirets experiments. The directories are arranged first by cell type, then by the date of the experiment for that cell type. Each trial has two text files, one that features that raw data of fly position and one that features the synchronization data between the imaging frames and the visual display (noted with "_SYNC").Analysis code can be found at https://github.com/DanTurner-Evans/BiologicalRingAttractor-CodeRelated materials in the collection (https://doi.org/10.25378/janelia.c.5179721):Neuron skeletons and synapses: https://doi.org/10.25378/janelia.12497756One color calcium imaging data: https://doi.org/10.25378/janelia.12490373Kir perturbation calcium imaging data: https://doi.org/10.25378/janelia.12490325Shi perturbation calcium imaging data: https://doi.org/10.25378/janelia.12490313Two color calcium imaging data: https://doi.org/10.25378/janelia.12490274Dataset supports the publication:Turner-Evans, D. B., Jensen, K. T., Ali, S., Paterson, T., Sheridan, A., Ray, R. P., Wolff, T., Lauritzen, J. S., Rubin, G. M., Bock, D. D., & Jayaraman, V. (2020). The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor. Neuron, 108(1), 145-163.e10. https://doi.org/10.1016/j.neuron.2020.08.006AbstractNeural representations of head direction (HD) have been discovered in many species. Theoretical work has proposed that the dynamics associated with these representations are generated, maintained, and updated by recurrent network structures called ring attractors. We evaluated this theorized structure-function relationship by performing electron-microscopy-based circuit reconstruction and RNA profiling of identified cell types in the HD system of Drosophila melanogaster. We identified motifs that have been hypothesized to maintain the HD representation in darkness, update it when the animal turns, and tether it to visual cues. Functional studies provided support for the proposed roles of individual excitatory or inhibitory circuit elements in shaping activity. We also discovered recurrent connections between neuronal arbors with mixed pre- and postsynaptic specializations. Our results confirm that the Drosophila HD network contains the core components of a ring attractor while also revealing unpredicted structural features that might enhance the network’s computational power.