Supplementary Data Figures: Antibodies validated for routinely processed tissue unpredictably stain frozen tissue sections

Supplementary dataset The folder contains individual antibody staining (~100) on frozen tonsil sections, fixed with acetone, 4% formaldehyde or antigen retrieved (AR) following formalin fixation. Each image is a stack of 3 TIFF unmodified fluorescence images on serial sections. Fixation and antibody name can be found in each image title. The collection is a compressed .zip file.The dataset can be downloaded at the Mendeley repository:Cattoretti, Giorgio; Bolognesi, Maddalena M; Bosisio, Francesca M; Faretta, Mario; Mascadri, Francesco (2020), “Antibodies validated for routinely processed tissue unpredictably stain frozen tissue sections.”, Mendeley Data, V1, doi: 10.17632/j88c2ftpsr.1http://dx.doi.org/10.17632/j88c2ftpsr.1References1. Sutherland BW, Toews J, Kast J. Utility of formaldehyde cross-linking and mass spectrometry in the study of protein-protein interactions. Journal of mass spectrometry : JMS, 43(6), 699-715 (2008).2. Bolognesi MM, Manzoni M, Scalia CR et al. Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections. Journal of Histochemistry & Cytochemistry, 65(8), 431-444 (2017).3. Scalia CR, Boi G, Bolognesi MM et al. Antigen Masking During Fixation and Embedding, Dissected. Journal of Histochemistry & Cytochemistry, 65(1), 5-20 (2017).