Functional convergence of growth responses to shade and warmth in Arabidopsis

The goal of this study was to compare the transcriptional profile (RNA-seq) of Arabidopsis thaliana Columbia-0 ecotype seedlings that were treated with two different light (white light or shade) and temperature (20ºC or 28ºC) conditions. Seeds of Arabidopsis thaliana were sown on 0.8 % agar-water in Petri dishes, stratified 5-7 d at 5 ºC in darkness and transferred to a growth room at 22°C with a photoperiod of 10 h of 100 μmolm −2 s −1 of white light (400–700 nm) provided by a mixture of fluorescent and halogen lamps (red/far-red ratio = 1.1). Three-day-old seedlings of the wild type Columbia were exposed during the photoperiod of day 4 (10 h) to white light at 20°C, white light at 28°C, shade at 20°C or shade at 28°C. Three biological replicates of each condition were collected at the end of the photoperiod of the day 4.