Leptospirosis mixed infections

The aim of this study was to obtain clonal isolates from the mixed populations of pathogenic Leptospira species cultured from the nine infected rodents. A combination of selective approaches and plating on HAN agar at 37C enabled the separation and recovery of different species that can be characterized to further understand how mixed infections contribute to the epidemiology of leptospirosis and their role in the transmission of infection to humans and domestic animals.