Uncover Genetic Networks Controlling Fruit Quality in Two Strawberry Cultivars

Strawberry fruit quality, particularly texture and sweetness, is governed by complex molecular mechanisms during ripening. This study investigated two varieties with contrasting phenotypes: 'Monterey', a firm-fleshed commercial cultivar, and 'Three Princess', a softer, sweeter variety. We analyzed five key developmental stages (green, white, color-change, red, and overripe) to unravel the transcriptional basis of their divergent quality traits. RNA-seq revealed extensive differential gene expression (11,382 DEGs at the red stage), with 'Three Princess' showing earlier activation of starch/sucrose metabolism and polygalacturonases, correlating with its rapid softening and higher soluble solids content (SSC).