Identifying the Role of Zuo1 protein in Saccharomyces cerevisiae. Identifying the Role of Zuo1 protein in Saccharomyces cerevisiae

The ribosome associated complex (RAC) is a ribosome bound protein chaperone complex reported to surveil the translation of proteins with positively charged regions. It has been posited that RAC might be able to directly regulate translation by coupling co-translational folding with the peptide-elongation cycle. To identify the targets of RAC in cells and test the hypothesis that the complex modulates translation, we performed ribosome profiling on wild- type yeast cells and cells lacking a key component of the RAC that binds near the ribosome active site (zuo1Δ). Overall design: mRNA-seq and Ribosome profiling of exponentially growing (OD600 = 0.6) Saccharomyces cerevisiae cells were performed by deep sequencing , in two biological replicates, using Illumina HiSeq-4000 platform.