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Identification of miR-21 targets in Jurkat cells by AGO2 immunoprecipitation

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE37212
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In order to identify miR-21 targets by a biochemical high-throughput method, we immunopurified RISC Complex and associated mRNAs in both control and miR-21 overexpressing Jurkat cells. Following transduction of miR-21 encoding construct (or a cognate control vector) in Jurkat cells, we used microarray technology to profile Total RNA, AGO2 Immunopurified RNA and control IgG purified RNA.
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2020-05-29
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