Comparison of the Effectiveness of Core Genome Multilocus Sequence Typing and Polymerase Chain Reaction-Based Open Reading Frame Typing in Tracing Nosocomial Methicillin-Resistant Staphylococcus aureus Transmission

We performed polymerase chain reaction-based open reading frame typing (POT) and core genome multilocus sequence typing (cgMLST) to investigate the occurrence of nosocomial transmission of MRSA and to compare the results of these methods.POT and whole genome sequencing were performed in 83 MRSA isolates.The cgMLST proved more effective for identifying nosocomial transmissions compared to POT, highlighting its utility in tracking MRSA spread in healthcare settings.