DNA metabarcoding of grapevine Ascomycota community

The project aimed to study the effect of drought and esca expression on grapevine Ascomycota community using DNA metabarcoding. We collected 251 grapevine samples: 48 petioles, 47 stems of the current year, 39 canes, 117 trunk samples (43 healthy wood, 36 black necrosis, 29 white rot, and 9 brown stripes) at the end of the experiment presented in Bortolami et al. (2021; https://doi.org/10.1073/pnas.2112825118). Plants were mature 30-y-old Vitis vinifera cv. Sauvignon blanc from three treatments: well-watered control asymptomatic and esca symptomatic vines, and asymptomatic vines under water deficit. We realized fungal GTAA amplification (primers GTAA182f and GTAA526r; Morales-Cruz et al. 2018) and Illumina MiSeq sequencing. All the methods and results are presented in the following publication: Gastou, P., Bortolami, G., Ferrer, N., Gambetta, G.A., Moretti, S., Vallance, J. and Delmas, C.E.L., 2025. Differential impacts of drought and esca expression on Ascomycota fungi in the trunks and young organs of mature grapevines. bioRxiv, pp.2025-07. https://www.biorxiv.org/content/10.1101/2025.07.03.663063v1 Contact: Chloé Delmas (INRAE)