The mechanism of FSTL3 in the progression of colon cancer

FSTL3 is a multifunctional protein, and its role in cancer has attracted considerable attention. Firstly, overexpression of FSTL3 has been found to be closely associated with the occurrence and development of various tumors. The expression levels of FSTL3 are significantly elevated, which is linked to its role in promoting tumor cell proliferation, migration, and invasion. Additionally, the expression levels of FSTL3 are associated with the grading and prognosis of tumors, where high expression of FSTL3 is often correlated with the malignancy and adverse prognosis of tumors. Therefore, this study primarily explores the mechanisms by which FSTL3, acting as an exosomal cytokine, promotes the progression of colon cancer. Overall design: In order to investigate the mechanistic role of FSTL3 in the progression of colon cancer, the study involved the exogenous addition of humanized FSTL3 protein to the culture medium of HCT116 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different cells after 24 hours. This aimed to explore its impact on the biological functions of colon cancer cells and underlying mechanisms.