Control of amino acid content in plants
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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(i) comprising, consisting of, a sequence having at least 80% sequence identity with SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, or 15 , A polynucleotide consisting essentially of; (ii) a polypeptide encoded by the polynucleotide described in (i); (iii) at least 95% sequence identity with SEQ ID NO: 6 or SEQ ID NO: 8, at least 93% sequence identity with SEQ ID NO: 2 or SEQ ID NO: 10 or SEQ ID NO: 12, or at least 94 with SEQ ID NO: 4 or SEQ ID NO: 14 or SEQ ID NO: 16 A polypeptide comprising, consisting of, or consisting essentially of a sequence having% sequence identity; Or (iv) a construct, vector or expression vector comprising the isolated polynucleotide described in (i); a plant cell is described, wherein the plant cell is the expression or activity of a polynucleotide or a polypeptide It contains at least one modification that modulates the expression or activity of the polynucleotide or polypeptide compared to this unmodified control plant cell. The present invention discloses the polynucleotide sequences of genes encoding aspartate transaminase (AAT) from Nicotiana tabacum and the modulation of their expression. There is described a plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 80% sequence identity to SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13 or SEQ ID NO: 15; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence having at least 95% sequence identity to SEQ ID NO: 6 or SEQ ID No: 8, at least 93% sequence identity to SEQ ID NO: 2 or SEQ ID NO: 10 or SEQ ID No: 12, or at least 94% sequence identity to SEQ ID NO: 4 or SEQ ID NO: 14 or SEQ ID NO: 16; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), wherein said plant cell comprises at least one modification which modulates the expression or activity of the polynucleotide or the polypeptide as compared to a control plant cell in which the expression or activity of the polynucleotide or polypeptide has not been modified. The present invention discloses the polynucleotide sequences of genes encoding aspartate transaminase (AAT) from Nicotiana tabacum and the modulation of their expression. There is described a plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 80% sequence identity to SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13 or SEQ ID NO: 15; (ii) a polypeptide encoded by the polynucleotide set forth in (i); (iii) a polypeptide comprising, consisting or consisting essentially of a sequence having at least 95% sequence identity to SEQ ID NO: 6 or SEQ ID No: 8, at least 93% sequence identity to SEQ ID NO: 2 or SEQ ID NO: 10 or SEQ ID No: 12, or at least 94% sequence identity to SEQ ID NO: 4 or SEQ ID NO: 14 or SEQ ID NO: 16; or (iv) a construct, vector or expression vector comprising the isolated polynucleotide set forth in (i), wherein said plant cell comprises at least one modification which modulates the expression or activity of the polynucleotide or the polypeptide as compared to a control plant cell in which the expression or activity of the polynucleotide or polypeptide has not been modified.
提供机构:
国家林业和草原科学数据中心
创建时间:
2023-02-12



