Identification of SMXL4- and SMXL5-dependent genes in comparison to the SMAX1-dependent genes

We used RNA-seq to generate the transcriptional profile of 7 days old WT, smxl5-1, smxl4-1 smxl5-1 and smax1-2 Arabidopsis mutant seedlings. We then used statistical analysis to identify differentially expressed genes that potentially contribute to the smxl5-1, smxl4-1 smxl5-1 and smax1-2 mutant phenotype. RNA from three replicates each of WT and smxl5-1, smxl4-1 smxl5-1 and smax1-2 mutants were sequenced and differentially expressed genes were identified by using the DESeq2 package.