Transcriptome analysis of KM-H2 cells with different Rhodamin 123 eflux capacity. Transcriptome analysis of KM-H2 cells with different Rhodamin 123 eflux capacity

Rhodamin 123 is a dye which can be used to detect the activity of ABC transporters. We observed that after staining of KM-H2 Hodgkin lymphoma cells with Rhodamin 123, part of the cells rapidly eliminated the dye, while another part of the cells retained the dye for a longer time. We compared the transcriptome of KM-H2 Hodgkin lymphoma cells with high Rhodamin 123 efflux capacity and KM-H2 cells with low Rhodamin 123 efflux capacity. Overall design: KM-H2 cells were stained with Rhodamine 123, washed, and incubated for 4 hrs. Thereafter, cells with high Rhodamine 123 efflux capacity and low efflux capacity were separated by flow cytometry. Samples were analyzed using the Clariom D Human platform. Array data was processed by using Transcriptome Analysis Console.