Comparison of EM studies with Mediator and RNA polymerase II.
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aThe number of single-particle images included in the final angular refinement: the structurally homogenous data set.bPol II in this sample was missing RPB4 and RPB7.cYeast Mediator head module.dDifferent subsets of the data could be used that yielded similar structures with identical pol II docking results.eParticle number includes free VP16-Mediator, which was also present in these samples and was refined independently (e.g. see Figure S3).fTwo distinct Mediator–pol II substructures were observed in the absence of TFIIF.
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2015-12-02
