Hepatic transcriptome analysis of WT and PRDX1Cys52Ser mutant mice on CDAHFD

In this study, we investigated how the peroxidase peroxiredoxin 1 protects against NASH. One previous study showed that PRDX1 cys52 was mutated to ser52 and consequently PRDX1's peroxidase activity was impaired. Surprisingly, PRDX1Cys52Ser mice showed robust resistance to diet-induced NASH. To this end, We sought to understand how PRDX1Cys52Ser mutant confers resistance to NASH although impairing PRDX1's peroxidase activity. We fed WT and PRDX1Cys52Ser mice a CDAHFD for 8 weeks and collected liver samples for RNA sequencing analysis. As revealed by gene-set enrichment analysis (GSEA) and KEGG pathway enrichment analysis, there was an enrichment of genes involved in inflammation, fiberosis and JAK-STAT signaling. Overall design: We fed 8-week-old male mice a CDAHFD for 8 weeks and collected their liver samples for RNA sequencing. 3 mice per genotype (WT or PRDX1 Cys52Ser) were used.