RNA-seq identifies lncRNAs in hyperreactive and hyporeactive platelets

Platelets are blood cells who play critical roles in numerous biological and disease processes. This study was designed to identify lncRNAs that may play a role in platelet reactivity. In this study, by using large-scale deep sequencing, we determined the expression profiles of lncRNAs in both hyperreactive and hyporeactive human platelets. To determine the potential link between the expression of lncRNAs and the function of platelets, the expression profiles of hyperreactive and hyporeactive platelets were compared. Compared with hyperreactive platelets, deep sequencing analysis demonstrated that differential lncRNA expression was a remarkable characteristic in hyporeactive platelets. Overall design: To determine the expression profiles of lncRNAs and mRNAs in platelets, RNAs from both hyperreactive and hyporeactive platelets were used. Three micrograms of RNA per sample were used as the input material for the RNA sample preparations. The expression profiles of lncRNAs and mRNAs were determined by genome-wide large-scale next-generation deep sequencing by using the Illumina platforms.