A transcriptomic characterization of the molecular mechanisms induced by RGMa during skeletal muscle hyperplasia and hypertrophy. Mus musculus strain:C3H | cultivar:C2C12

Repulsive guidance molecule a (RGMa) was originally identified as a repulsive clue in the orientation of axonal growth in the central and peripheral nervous system and as an important target for neuronal survival. However, RGMa action domains were found to go beyond the processes related to neurogenesis and could be extended to different processes, including myogenesis. Our previous works have consistently shown that RGMa is expressed in skeletal muscle cells and that its overexpression induces both nuclei accretion and hypertrophy in muscle cell lineages. However, the biologycal processes, cellular components and molecular mechanisms regulated by RGMa during the differentiation of skeletal muscle cells are poorly understood. In this work we aim to investigate, through RNA-seq analys, the molecular mechanisms modulated by this axon guidance molecule during myogenic differentiation of recombinant-RGMa treated C2C12 cell.