RNA-seq reveals differential expression of potential miR-486 target transcripts

Identifying microRNA target transcripts is critical in understanding their role in modulation of disease pathology through cellular processes. Here, we used isolated 6 month mouse tibialis anterior (TA) muscle tissue for RNA extraction and sequencing in order to identify mRNA transcripts overexpressed in miR-486 knockout mice compared to wildtype. Tibialis anterior muscle transcriptomic profiles of 6 month male miR-486 knockout (KO) and wildtype (WT) mice were generated in triplicate.