Transcriptome profiling of vascular fragments at the preclinical, progression and remission phase of EAE in imatinib and PBS treated mice

Disruption of blood-brain barrier (BBB) integrity is a hallmark of several neurological disorders. Here we show that the BBB is dynamically and differentially affected during the preclinical, progression and remission phase of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). We isolated vascular fragments, representing the BBB, from spinal cords of imatinib and PBS treated mice at the preclinical, progression and remission phase of MOG-EAE as well as from non-immunized, naive mice. We analysed the transcriptome of vascular fragments of mice treated with imatinib or PBS at the preclinical, progression and remission phase of EAE (n=4 for all EAE time points and conditions), respectively, and of vascular fragments from healthy control mice (n=5, without EAE). We compared PBS versus imatinb and PBS versus naive for all time points.