File S1 - An Important Role of the SDF-1/CXCR4 Axis in Chronic Skin Inflammation

Figure S1, Inhibition of the SDF-1/CXCR4 axis decreases the number of several types of inflammatory cells in chronically inflamed skin. Single-cell suspensions from inflamed ear skin were analyzed by FACS for the presence of different inflammatory cell populations. (A, B) Inflamed ear skin was analyzed for the presence of CD3+/CD4+ and CD3+/CD8+ T-cells. AMD3100 and anti-SDF-1 treatment significantly decreased the number of CD4+ cells, as compared with control mice. Compared to PBS-treated mice, AMD3100 further significantly decreased the number of CD8+ cells in the inflamed ear skin. Anti-SDF-1 showed the same tendency. (C, D) The number of dendritic cells (DC) was investigated by analyzing I-A/I-E+CD11c+ cells. Inhibition of the SDF-1/CXCR4 axis resulted in significantly reduced numbers of DC in the inflamed ear skin. Anti-SDF-1 treatment also significantly decreased the number of CXCR4+ DC. AMD3100 treatment showed the same trend. (E, F) Macrophages were assessed by evaluating the CD11b and F4/80 double positive population within live CD45+ leukocytes. AMD3100 significantly reduced the number of macrophages as well as of CXCR4+ macrophages in the inflamed ear skin. Anti-SDF-1 showed a tendency to reduce the number of these inflammatory cell populations and significantly reduced the number of CXCR4+ macrophages in the inflamed ear skin. n = 5 per group. Two independent experiments were performed. Data represent mean ±SD. *P<0.05; ***P<0.001. ns, not significant.
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