Fig. A, 1H NMR spectra (400 MHz) for: (a) MeO-PEGN-SuccOH; (b) the resveratrol-PEG conjugate mixture from the synthesis of MeO-PEGN-Succ-RSV; (c) resveratrol; and (d) the solution from (b) after treatment with water and heat (90°C) for ca. 1 week).

All solutions are in d6-DMSO. Key: † = d5-DMSO, ‡ = H2O. To identify the low intensity down-field signals the spectra in (a), (b) and (d) have been expanded vertically. Fig. B, The down field region of the 1H NMR spectra (400 MHz) for: (a) a solution of the resveratrol-PEG conjugate mixture in d6-DMSO showing the assigned signals for the two major products 4'-MeO-PEGN-Succ-RSV and 3-MeO-PEGN-Succ—RSV; and (b) a solution of resveratrol in d6-DMSO. Fig. C, 1H NMR spectra for the MeO-PEGN-Succ-RSV conjugate mixture in d6-DMSO, recorded at 400 MHz. Key: † = d5-DMSO, ‡ = H2O. Fig. D, 1H NMR spectra for MeO-PEGN-Succ-OH in d6-DMSO, recorded at 400 MHz. Key: † = d5-DMSO, ‡ = H2O. Fig. E, 1H NMR spectra of the MeO-PEGN-Succ-RSV conjugate mixture after treatment with water and heat (90°C) for ca. 1 week, in d6-DMSO, recorded at 400 MHz. Key: † = d5-DMSO, ‡ = H2O. (DOCX)