The role of TIGIT in idiopathic inflammatory myopathies

To investigate the role of Tight in idiopathic inflammatory myopathies.We evaluated global transcriptome changes in the quadriceps muscles of Tight-/- and WT mice with EAE. For EAM induction, 6-week-old female BALB/c mice were immunized with 100 μl of 50% complete Freund’s adjuvant (Sigma, USA) containing 1mg myosin (or with an equal volume of PBS in control group) on bilateral sides of the hind foot pads, the tail base and flanks four times at 1-week intervals as previously described. Total RNA was isolated from the quadriceps muscles of Tigit-/- and WT mice with EAE