Molecular phylogenetic assessment of the genus <i>Scutellinia</i> (Pezizales: Pyronemataceae)

The genus <i>Scutellinia</i> consists of cup fungi belonging to the <i>Pyronemataceae</i> (Ascomycota). It comprises more than 100 species with a worldwide distribution. Species delimitation is based on morphological characteristics of the ascocarps, such as spore size, form and ornamentation as well as size and morphology of hairs that typically cover the external surface of the ascoma. These characters are, however, highly variable and species recognition challenging. In this paper, ribosomal DNA traits such as the internal transcribed spacer and the large subunit ribosomal gene were sequenced in several <i>Scutellinia</i> samples and, along with all available sequences on public database, used to improve the knowledge about taxonomy and phylogeny of this genus. Based on sequence similarity and trees topology, about 50 phylogenetic species were recognized and their phylogenetic relationships disclosed.盾盘菌属（*Scutellinia*）隶属于子囊菌门（Ascomycota）火丝菌科（Pyronemataceae），为一类杯状真菌。该属目前已报道超过100个物种，呈全球分布格局。传统物种界定主要依据子囊果（ascocarps）的形态特征，包括孢子大小、形态与纹饰，以及通常覆盖于子囊果外表面的附属毛的尺寸与形态。然而此类性状存在高度变异，使得物种识别颇具挑战性。本研究对多份盾盘菌属样本的核糖体DNA序列进行测序，所检测的分子标记包括内转录间隔区（internal transcribed spacer）与大亚基核糖体基因，并结合公共数据库中已公开的全部相关序列，以深化对该属分类学与系统发育的认知。基于序列相似性与系统发育树拓扑结构，本研究共识别出约50个系统发育种，并阐明了它们之间的系统发育关系。

搜集汇总

背景与挑战

背景概述

该数据集聚焦于Scutellinia属（一种杯状真菌）的分子系统发育研究，通过分析核糖体DNA序列（ITS和LSU）来改进物种分类和亲缘关系识别。研究基于序列相似性和系统树拓扑，识别了约50个系统发育物种，旨在解决传统形态学特征变异大、物种识别困难的问题。数据集包含原始实验数据和相关文档，发布于2022年，涉及微生物学、遗传学和进化生物学等多个学科领域。