Dynamics of Subcellular Proteomes During Brain Development

Many neurological disorders are caused by perturbations
during
brain development, but these perturbations cannot be readily identified
until there is comprehensive description of the development process.
In this study, we performed mass spectrometry analysis of the synaptosomal
and mitochondrial fractions from three rat brain regions at four postnatal
time points. To quantitate our analysis, we employed 15N labeled rat brains using a technique called SILAM (stable isotope
labeling in mammals). We quantified 167429 peptides and identified
over 5000 statistically significant changes during development including
known disease-associated proteins. Global analysis revealed distinct
trends between the synaptic and nonsynaptic mitochondrial proteomes
and common protein networks between regions each consisting of a unique
array of expression patterns. Finally, we identified novel regulators
of neurodevelopment that possess the identical temporal pattern of
known regulators of neurodevelopment. Overall, this study is the most
comprehensive quantitative analysis of the developing brain proteome
to date, providing an important resource for neurobiologists.