Overview of the number of animals used in protocol 2; in addition, the number of slides (D40 distribution) that were analyzed is given.

In protocol 2 data were obtained in the same rats during baseline conditions and during insulin administration. Protocol 2a involved 4 different groups of rats; for the insulin condition hindlimb muscles from a subset of rats that underwent the IVITT in protocol 2 were used (indicated by *). Furthermore, additional rats (n = 3) were used in this protocol to measure the contribution of autofluorescence (see Methods).