Genome wide maps of ELK1 bingding sites in HDFs control and mechanic loading groups.

HDFs were cultured for 24 hrs for adhesion and to reach 50-70% confluence before mechanical tension was applied. Cyclic mechanical stretch (CMS) with a 0.5-Hz sinusoidal curve at 10-20% elongation was applied using a Flexcell○RFX-5000TM Tension System during the stretching. Cells were harvested immediately after the application of CMS stimulation was completed. Control cells were cultured on the same plates in the same incubator with no stretching. Examination of ELK1's different bind motifs of HDFs in the control condition and mechanic loading condition.