Directly converted astrocytes retain the ageing features of the donor fibroblasts and elucidate the astrocytic contribution to human CNS health and disease.

To interrogate the transcriptional features of fibroblast-derived iAstrocytes in relation to their ageing phenotype, we compared transcriptomic data from iAstrocytes obtained from old and young donors to bona fide aged human astrocytes laser captured from post-mortem (PM) brains and fetal primary astrocytes.Research Ethics:Study number STH16573, Research Committee reference 12/YH/0330 and MODEL-AD research study number STH19080 Research and Ethics Committee number: 16/YH/0155<br>