Frequent mutations in human lung cancer tumors detected by Ion Torrent DNA sequencing

In order to identify genetic mutations in individual human lung cancer, we sequenced 13,500 loci from 45 cancer-related genes in 82 human lung cancer samples using the Ion Torrent Ampliseq Cancer Panel. The sequencing analysis revealed frequent missense mutations, unique combination mutations, and hotspot loci in the genes BRAF, EGFR, K-ras, PIK3CA, and TP53 in the human lung cancer samples of various histologic types.