Image repository from : Subcellular nanoparticle trafficking investigated with label-free, live cell imaging

Nanoparticle drug delivery systems have significant potential to transform precision medicine due to their ability to encapsulate a wide range of cargos that can be packaged in various types of carriers. Delivery kinetics can also be modified by altering the surface chemistry of the nanoparticle carrier. However, a gap remains in our understanding of the cellular mechanisms underlying nanoparticle uptake and subcellular trafficking kinetics. A comprehensive understanding of nanoparticle-cell interactions considering both the nanocarrier and the drug cargo has been challenging, partly due to technological limitations. Here, we present a robust long-term imaging workflow as a novel tool to study live-cell dynamics and nanoparticle delivery. We show how the integration of holography, tomography, and fluorescence microscopy enhances the capacity to study living, adherent cells in vitro label-free with further applications in assessing nanoparticle uptake and delivery kinetics over time. We ..., , # Image repository from : Subcellular nanoparticle trafficking investigated with label-free, live cell imaging Dataset DOI: [10.5061/dryad.zkh1893qf](https://doi.org/10.5061/dryad.zkh1893qf) ## Description of the data and file structure The contents of this Dryad dataset include all the acquisition files, edited montages generated using ImageJ, auto-adjusted processed acquisitions with added scale bars, and the metadata cell sheet, which describes the acquisition parameters set for the corresponding data file. <br /> The files are all TIFF files. These data are titled to indicate as much detail as necessary to identify the file. This includes: the format of the image (movie, still, Combined Stack, Merged, or montage), the cell line type (SKNBE2, T47D, or LOXIMVI as “LOX”), the treatment type (bare liposomes, LNPs, UT=Untreated, Doxil, or free dox[orubicin]), the durations (in hours) and/or what the scale bar is in microns (“um”). Further information in the titles may include the tr..., ,