Single Cell Profiling Identifies the Histone Demethylase, JMJD3, Modulates Macrophage Phenotype in Abdominal Aortic Aneurysm Formation

Abdominal aortic aneurysm (AAA) is a life-threatening disease characterized by an inflammatory macrophage phenotype that contributes to pathological vascular remodeling. Although the mechanisms regulating macrophage-mediated inflammation during AAA development remain undefined, accumulating evidence suggests that epigenetic alterations play a critical role. Here using single cell RNA-sequencing techniques, we identify that the histone demethylase JMJD3 is elevated in human AAA monocytes/macrophages, resulting in the upregulation of cellular activation and a proinflammatory immune response. Overall design: scRNA-seq for AAA