Gene expression profiles of differentially expressed genes in Enterococcus faecalis 14 ΔpnpA mutant strain. Gene expression profiles of differentially expressed genes in Enterococcus faecalis 14 ΔpnpA mutant strain

To explain the particular behavior of the mutant ΔpnpA strain, we performed a comparative transciptomic analysis of RNA isolated from a derivative strain ΔpnpA mutant versus the wild-type (WT) , after 3h and 6 h of growth in GM17 medium under semi-aerobic conditions. Overall design: Expression data of the 3 wild-type samples were filtered and the average was calculated for each gene, for 3h and also for 6h condition. A fold change (FC) value was calculated between ΔpnpA individual samples and the mean of WT for each point of kinetics. Differentially expressed genes (DEGs) were selected for a FC threshold > 2.0 or < 0.5. Please note that the gpr files contains raw data obtained from the 4-plex/block slides and the .xlsx file (linked to each sample) contains the corresponding sample raw data (obtained from the selected block).