Biochanin A, an isoflavone isolated from Dalbergia sissoo Roxb. ex DC., leaves promote ROS-mediated and caspase-dependent apoptosis in lung adenocarcinoma cells

The objective of this study was to isolate and characterize a cytotoxic compound from the hydromethanolic extract of Dalbergia sissoo Roxb. ex DC. leaves using the cold percolation technique. Thin-layer chromatography was employed to isolate the cytotoxic component from the crude plant extract, and its cytotoxicity against lung adenocarcinoma (A549) cells was evaluated using the MTT assay. The structure of the isolated cytotoxic compound was determined through FTIR, NMR, UV analysis, and LC-MS/MS methods. Through comprehensive characterization, a cytotoxic compound called Biochanin A (BA) was identified, exhibiting significant anticancer activity with an IC50 value of 21.92 ± 2.19 μM against A549 cells, while demonstrating lower cytotoxicity towards normal lung cells (WI-38) with an IC50 value of 285.12 ± 2.19 μM. Notably, BA induced morphological changes in A549 cells, leading to apoptotic alterations and the generation of reactive oxygen species (ROS), as confirmed by multiple techniques (AO/EB, DAPI, Giemsa). In silico molecular docking, ADMET, MMGBSA, and molecular dynamics simulation investigations support the RT-PCR and cell biology findings. As a result, BA’s molecular mechanism of action involves ROS-induced apoptosis mediated by caspases 9 and 3. Isolation of a bioactive compound was accomplished through the utilization of thin-layer chromatography, using hydromethanolic leaves extract of Dalbergia sissoo Roxb. ex DC.Structural elucidation of the bioactive compound was conducted employing various methods, including UV analysis, FTIR, NMR, and LC-MS/MS analysis.In silico molecular docking, ADMET, MMGBSA, and molecular dynamics simulation investigations support the RT-PCR and cell biology findings.The investigation revealed a plausible mode of action, demonstrating that Biochanin A possesses the capability to induce apoptosis specifically in lung cancer cells (A549). Isolation of a bioactive compound was accomplished through the utilization of thin-layer chromatography, using hydromethanolic leaves extract of Dalbergia sissoo Roxb. ex DC. Structural elucidation of the bioactive compound was conducted employing various methods, including UV analysis, FTIR, NMR, and LC-MS/MS analysis. In silico molecular docking, ADMET, MMGBSA, and molecular dynamics simulation investigations support the RT-PCR and cell biology findings. The investigation revealed a plausible mode of action, demonstrating that Biochanin A possesses the capability to induce apoptosis specifically in lung cancer cells (A549).