Genome-wide analysis of transcription factor PPARgamma-responsive gene expression in the nodose ganglion (NG)

Analysis of gene expression regulated by PPARgamma in the nodose ganglion of Phox2b::Cre; PPARgamma fl/+ and Phox2b::Cre; PPARgamma fl/fl mice. Results demonstrate potential PPARgamma transcriptional targets in neurons RNA was purified from laser-captured nodose neurons. Three independent biological replicates were prepared by pooling RNA of nodose neurons from multiple animals of the same genotypes. Genomics and Microarray Core Facility at UT Southwestern ( http://microarray.swmed.edu/) checked RNA quality and performed the hybridization with a Mouse-6 V2 BeadChip (Illumina Inc.). We used Partek Genomics Suite 6.5 (Partek Inc.) and Ingenuity Pathway Analysis (Ingenuity Systems Inc.) for data and pathway analysis respectively.