Specificity of TaqMan PCR and conventional PCR for <i>F. verticillioides, F. proliferatum</i> and other sugarcane fungal pathogens (<i>Sporisorium scitamineum</i> and <i>Phoma</i> sp.<i>)</i> and endophyte (<i>Acremonium</i> sp.).
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For quantitative TaqMan PCR reactions, two primer-probe combinations were used; FAM-gx1 is specific to F. verticillioides; FAM-gx2 specific to F. proliferatum. For conventional PCR, two primer sets were used; Pgx1 is specific to F. verticillioides; Pgx2 specific to F. proliferatum. ‘+’ is positive; ‘−’ is negative.
创建时间:
2014-08-20



