Identification of proteins differentially associated with SARS CoV-2 subgenomic RNAs

We characterize the specific protein interactomes of 3 SARS sgRNAs (S, N, and ORF8) as well as the gRNA at two time points, 8- and 24- hours. These two timepoints were selected to capture early viral infection when RNA synthesis is just beginning, and viral replication is being established, as well as a later stage where protein synthesis and virion packaging is occurring. We identified over 500 proteins associated with one or more of the viral RNAs. These include both host and viral proteins that have been previously shown to bind to SARS-CoV-2 RNAs and novel (not previously identified) protein interactors.