RNA binding regulates enzymatic activity and chromatin binding of the nucleosome remodeler dMi-2/CHD4

The ATP-dependent nucleosome remodeler Mi-2/CHD4 modulates epigenetic landscapes to regulate gene expression and to maintain genome integrity. Here we use individual nucleotide resolution crosslinking and immunoprecipitation (iCLIP2) to show that Drosophila Mi-2 associates with thousands of mRNA molecules in vivo. Biochemical data reveal that recombinant dMi-2 preferentially binds to G-rich RNA molecules using two intrinsically disordered regions of previously undefined function.