Re-expression of SMARCA4/BRG1 in Small Cell Carcinoma of Ovary, Hypercalcemic Type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism [COV434_RNA-seq]
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https://www.ncbi.nlm.nih.gov/sra/SRP299935
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COV434 cells +/- BRG1 re-expression - RNA-seq Overall design: Three biological replicates of COV434 cells were co-transfected (FuGENE 6, Promega #E269A) with control (pCDNA3-neo) or BRG1 (PBJ5-BRG1) plasmids at a 10-6.25:1 ratio to a plasmid containing puromycin resistance (pBABE-puro). Twenty-four hours after transfection, cells were placed on 2-3 µg/ml puromycin for 3 days and then cell pellets were harvested for RNA-seq.
创建时间:
2021-04-03



