Gene expression analysis of THP-1 cells cultured with or without BCAA

The branched-chain amino acid (BCAA) metabolism plays pleiotropic roles in homeostasis. Here we show that human acute leukemia-initiating cells (LICs), but not normal hematopoietic stem cells, are heavily addicted to the BCAA metabolism, irrespective of myeloid or lymphoid types. To clarify how BCAA metabolism affect the gene expression of human acute leukemia cells, we examined the gene expression alteration in human acute leukemia cell lines in control and BCAA-resrticted culture conditions. To clarify the global gene expression alteration in AML cells after BCAA metabolism inhibition, THP-1 cells were cultured with or without BCAA. After 3-5 days of culture, FACS-purified THP-1 cells were subjected to transcriptome analysis.