Transcriptomic analysis of human naive B cells stimulated by CD40L, CpG, anti-IgM IgG, IL-4 or combinations

B-lymphocytes play major adaptive immune roles, producing antibody and driving cell mediated responses. However, how B-cells acutely differentiate in response to receptor signaling codes, including T-cell dependent versus independent cues, remains incompletely understood. To gain insights, we used multi-omic profiling to characterize ex vivo primary human B-cell transcriptomic, proteomic and metabolomic remodeling by B-cell receptor (BCR), Toll-like receptor 9 (TLR9), CD40-ligand (CD40L), interleukin-4 (IL4) or combinations thereof, highlighting key stimulus-specific phenotypes. Primary human B-cells were treated in culture in the absence of stimulation or stimulated by, CD40L only, CpG only, IL4 only, aIgM only, CD40L+CpG, CD40L+IL4, CpG+ aIgM, CD40L+ aIgM, and CD40L+ aIgM+IL4. Cells were treated for 24 hours. For each experiment, cells from 3 donors were isolated and treated separately. Primary human B-cells knocked out for BCAT1.