Transcripts with H3K36me3 modification at differente stages of pancreas organogensis [CUT&Tag]

To find the genes with H3K36me3 modificaiton at different stages of pancreas development, we isolated the pancreatic epithelial cells from E11.5, E13.5 and E15.5 fetal pancreas. Considering the low expression of H3K36me3 at E11.5, E11.5 samples worked as a "negantive" control. By compared with the peaks of E11.5, we was able to find genes under direct regulation of H3K36me3 at E13.5 and E15.5. Overall design: Pancreatic epithelial cells were purified from fetal pancreas at E11.5, E13.3 and E15.5 mouse pancreas, labelled by EpCAM and then sorted by flow cytometry and finally were subject to H3K36me3 CUT&Tag seq.