Effects of olive oil and oleic acid-derived endocannabinoidome mediators on food preference and high fat-induced obesity. Mus Musculus

Through a collaboration established with the Institute of Biomolecular Chemistry of the Italian National Research Center (ICB-CNR) thanks to the Unite Mixte Internationale on Chemical and Biomolecular studies between Laval university and the CNR, mice were fed either chow, or a high fat diet (HFD) or an isocaloric high fat diet but with olive oil instead of lard (HFDoil). HFD mice were also treated with synthetic OlGly and OlAla (50 mg/kg, i.p., every other day), two endogenous oleic acid-derived lipoaminoacids belonging to the endocannabinoidome. Mice were used in each experimental group. Food intake and body weight gain were measured weekly. At the end of the treatments, intestinal tissues were analysed for their microbiota composition by sequencing of 16S ribosome-encoding DNA (at IUCPQ) and endocannabinoidome mediator levels by LC-MS-MS (at ICB-CNR). The latter were also measured in the brain.