Identifying ASCL1 target genes in primary GBM stem cell cultures [ChIP-seq]

ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify genomic targets of ASCL1 in primary human GSC cultures. In this dataset, we include ChIP-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential ASCL1 binding between control and GSC cultures induced to overexpress ASCL1 after 18 hours of doxycycline treatment. Overall design: Glioblastoma cells with a genetic knockout of ASCL1 is compared to genetic knockout of ASCL1 overexpressing ASCL1 (n = 3 biological replicates; n = 2 input samples; total = 8 samples)