Pixel-wise Reflectance Spectra of Ex Vivo Porcine Tissues via Hyperspectral Imaging (500–5000 nm)

This dataset contains pixel-wise reflectance spectra (500-5000 nm) extracted from annotated hyperspectral (HS) images of ex vivo porcine tissues. The dataset was generated to support the study “Broadband Spectral Characterization of Ex Vivo Porcine Tissues via Hyperspectral Imaging across 500–5000 nm.”

The data can be used to investigate tissue-specific spectral signatures over a wide wavelength range. Note that full hyperspectral cubes are not included in this dataset, but may be shared upon request. The dataset also includes curated visual and spectral summaries designed to facilitate data exploration, comparison across systems, and reproducibility of the reported analyses.

Shared material organization

The released material is organized in two parts:

1. Data and metadata

By data, we mean the reflectance spectra extracted from annotated Regions of Interest (ROIs) of hyperspectral images. HS images were acquired using three complementary systems: visble near-infrared (VNIR, 500-1000 nm), short-wave infrared (SWIR, 1000-2500 nm), mid-wave infrared (MWIR, 2500-5000nm). Note that only pixel-wise spectral information is shared, whereas spatial relationships between pixels are not retrievable with these data. Each observation is a vector, corresponding to a single pixel reflectance spectrum.

The following elements are included:

Spectral reflectance data

Wavelength reference vectors

Metadata associated with each observation in the spectral reflectance data

Data are organized by spectral range:

VNIR

SWIR

MWIR

2. Data visual inspection

Supplementary material is provided to facilitate qualitative exploration of the dataset. For each analysed tissue sample, PDF reports are provideds. These contain:

Representative reflectance images (central wavelength slice of each HS cube)

Annotated ROIs used for spectral data extraction

ROI-averaged reflectance spectra

ROI-averaged first-derivative reflectance spectra

Supplementry material is grouped according to the following structure:

7 tissue classes: brain, adipose tissue (subcutaneous, ventral abdominal wall), myocardium, kidney, liver, skin, and spleen

5 specimens per tissue class (e.g., Organ 1–5)

2 samples per specimen (c1, c2)

Data type: Images or Spectra

3 spectral ranges: VNIR, SWIR, MWIR

3 rotations: approximately 0°, 45°, and 90° (rotation around the optical axis)

2 repetitions: two consecutive acquisitions per rotation

For Images PDF files, visual representations of the samples are displayed at a representative wavelength. For Spectra PDF files, reflectance spectra and their first derivatives are reported.

Further details about the dataset structure and contents are provided in the accompanying README file.