Genomics_of_eprinomectin_resistance_in_the_parasitic_worm_Haemonchus_contortus

Parasitic nematodes like Haemonchus contortus cost the livestock industry billions of dollars every year through production losses and treatment costs. Parasite control in the dairy industry widely relies on eprinomectin, a drug that is not excreted in milk and hence, is licensed for use without withdrawal time. The growing prevalence of eprinomectin-resistant worm populations threatens milk production in certain area like France. To understand how parasite adapt to this drug, we have sampled and phenotyped three eprinomectin-resistant populations from French Pays-Basque. These populations were matched with three drug-susceptible isolates from the same location. Male and female worms from every isolate were subjected to RNAseq to delineate the transcriptomic differences owing to each drug susceptibility status. In addition, a pool of larvae was used for whole genome sequencing to investigate genome-wide allele frequency patterns in these populations.