Bulk RNA‑seq of IMR90 fibroblasts in IR‑induced senescence with siRNA knockdowns of CCND1, CDK4, CDK6 and controls

We profiled transcriptomes of IR‑induced senescent IMR90 fibroblasts subjected to siRNA‑mediated knockdown of CCND1, CDK4, CDK6 (and CDK4+6), alongside two non‑targeting controls. Transfections occurred on days 4/5 and 7/8 post‑IR; all samples were harvested day 10 for RNA extraction. Differential expression identifies kinase‑specific roles in sustaining inflammatory and DNA damage responses in senescence. Conditions (n=3 each): • Proliferating (No IR) • Senescent + NT1 • Senescent + NT2 • Senescent + CCND1 KD • Senescent + CDK4 KD • Senescent + CDK6 KD • Senescent + CDK4+CDK6 KD Harvest day 10 post‑IR/transfections