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Unclassified single-stranded DNA from meteorite-enriched cultures reveals genomic dark matter elements

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Zenodo2025-06-05 更新2026-05-26 收录
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This dataset contains 22 unique single-stranded DNA (ssDNA) sequences obtained from microbial cultures treated with meteorite-derived minerals, as described in the manuscript: *Unclassified single-stranded DNA from meteorite-enriched cultures reveals genomic dark matter elements* (submitted to Scientific Reports). The sequences were recovered via metagenomic assembly from ultra-low input DNA and exhibit no homology to known reference genomes. All contigs share a highly conserved C/T-rich 30-bp motif and fold into stable stem-loop secondary structures, consistent with genomic dark matter elements. Files included:- MT_SEQ_ONES.fasta — FASTA file containing 22 ssDNA sequences.- Assembly_Metadata.tsv — Metadata table (coverage, assembler, molecule type).- Readme.txt — Full dataset description and contact information. Assemblers used: MEGAHIT v1.2.9, SPAdes v3.15.5, IDBA-UD v1.1.3-7. Contact: Dr. Carlos López Ramón y Cajal – carlos.lopez.ramon.y.cajal@sergas.es Affiliation: Hospital Álvaro Cunqueiro / University Hospital Complex of Vigo, Spain

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2025-06-05
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