Real-time quantitative PCR analysis of expression profiles of dual-specificity phosphatase family members in mouse tissues

Mouse tissues (including heart, skeletal muscle, brain, lung, stomach, small instestine, colon, liver, kidney, testis, uterus, bone marrow, thymus, lymph node, spleen and embryo) were isolated from three C57BL/6 mice (eight-week-old, uterus from female and other tissues from male). We used Applied Biosystems 7500/7500 Fast Real-Time PCR Systems to quantitate expression levels of DUSP family members in the indicated mouse tissues. qPCR gene expression profiling. Various tissues pooled from three C57BL/6 mice were used as indicated in the summary. Equal amount total RNA from each tissue was pooled prior to gene expression analysis.